Retro-Tech
Gesellschaft für Retrovirale Technologie
D-85716 Unterschleißheim
Tel.: +49-89-32141960
Fax: +49-89-32141959
eMail: info@retrotech-online.de

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Classes Antiviral Screening Service Biological Safety & Quality Testing Service Bio Consulting Training Courses



Antiviral Screening Service

of

pharmaceuticals, combinatorials
chem. & biol. libraries,
natural extracts

by

inhibition of retroviral infection,
reverse transcription, maturation and assembly


  1. biochemical screening: by in vitro inhibition of

  2. cellular screening: by in vitro inhibition of

  3. characteristics:

  4. advantages:

  5. sample requirements:
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BioConsultTM

CorpConsultTM

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Biological Safety & Quality
Screening Service

for

Retrovirus Contamination


  1. Screening:
    of cell cultures, bio/pharmaceuticals, bio/materials, bio/cosmetics and bio/gentech-food for contamination with known or unknown retrovirus-particles, proteins or nucleic acids, recomb. vectors.

  2. Validation:
    of products and processes for inactivation, removal or concentration of retrovirus contaminants (biopharmaceuticals, filtration devices etc.).

  3. Characteristics:

    1. sample preparation: extraction or pelleting of potential retrovirus contaminants by
      • ultracentrifugation or precipitation.

    2. biochemical monitoring of retroviruses by
      • Reverse transcriptase i.e. RETRO-KIT/RTA Technology
      • Antigen ELISA`s
      • viral RNA hybridisation
      • Sedimentation analysis, density measurement
      • Morphological analysis by electronmicroscopy

    3. biological monitoring of retroviruses by
      • cellular infectivity assays (animal & human)


  4. Sample requirements:
    20-50 ml of sample (culture supernatant, sera etc.) cleared by centrifugation at 500xg (10 min) and 12.000xg (30 min). Information should be given on storage conditions, presence of mycoplasmal contamination, toxic or hazardous additives (e.g. sodium azide, protease inhibitors, metabolic or enzymatic inhibitors), organic solvents etc.
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Training

in

RTA - Reverse Transcriptase Technology (RT)


Training introduces the participants in the Reverse Transcriptase Technology, cost effective and easily. Handling of magnetic particles with the aid of the magnetic separator will be exercised as well.
Hand-outs including theoretical discourse and instruction manuals will be provided.
Participation will be documented by a training certificate.




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